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After the preparation of the culture medium and the inoculation of sterile seedlings is completed, you can then enter the last link of plant tissue culture-tissue culture seedling cultivation. The induction of new tissues needs to provide constant temperature and light conditions, and the continuous changes of external environmental conditions can not meet the normal growth of tissue culture seedlings, so light incubators and light culture racks came into being.
Both the constant temperature light incubator and the light culture rack can be used for the cultivation of tissue culture seedlings. The difference is that the light incubator can adjust the temperature and light intensity. At the same time, the cycle can be set according to the experimental needs. Carry out exploratory experiments on the basis of The advantage of the light culture rack is that the number of tissue culture seedlings is large. One light culture rack can hold 480 bottles of tissue culture seedlings at the same time, while the light culture incubator can only hold 150 bottles of tissue culture seedlings. Therefore, schools can choose appropriate experimental teaching equipment according to their teaching needs.
How to use constant temperature light incubator:
1. Open the outer package, find the matching key, and open the glass door of the light incubator.
2. The instrument is equipped with four trays, and the school can choose the appropriate number to install the trays according to the experimental use needs.
3. Tighten the connection device on the back of the instrument that controls the light.
4. Adjust the operation panel: In general, just set two cycles.
The first cycle parameter setting: light time 12h, light intensity 100%, temperature 25 ℃.
Parameter setting for the second cycle: light time 12h, light intensity 0%, temperature 18 ℃.
Note: The specific light time depends on the plant being cultivated.
How to use the light culture rack:
1. Assemble all parts of the light culture rack according to the instructions.
2. A timer is installed at the plug, and the unattended state can be realized after the setting is completed.
Setting parameters: open from 8: 00-18: 00; close from 18: 00-8: 00
3. Open the lamp to start cultivation.
Note: The light culture rack cannot adjust the outside temperature, so the air conditioner needs to maintain a constant temperature.
The ultra-clean workbench, high-pressure steam sterilization pot, light incubator and light culture rack, the perfect combination of several sets of instruments, make the original mysterious tissue culture experiment easy and simple. Since then, the plant tissue culture experiment has entered the middle school classroom. You who are eager to try quickly bring the students to realize the "beauty of flowers and leaves".
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